QGO-M XLPD™ - Quench-Gone™ Organic Modified XL Pre-Dilution

How and where using this product?

The QGO-M XLPD test kit is designed to measure biological activity in organic samples that are difficult to filter and/or have large quantities of inhibitory components, such as:

1. Latex/polymers emulsions
2. Personal care products
3. Concrete admixtures

The 2nd generation ATP measurement technology is the only one allowing interference-free quantification of living microorganisms in complex organic samples

Microbial growth in industrial organic chemicals can present a major problem. If left unchecked, microbial contamination in raw materials and intermediate products can compromise manufacturing process performance and equipment integrity. These same sources can also impact final product quality. The best solution is early, accurate detection for proactive rather than reactive treatment. When it comes to microbial risk management in industrial sector, QGO-M XLPD kit is the best tool to:

1. ensure the smooth operating of the production,

2. save money,

3. save time.

The QGO-M XLPD test kit utilizes a 3-minute filtration-based analysis to measure a parameter called cellular ATP (cATP™).

cATP represents ATP from living microorganisms in suspension in a fluid and therefore is a direct indication of the planktonic population.

Intracellular ATP (cATP), that is the ATP contained in living biological cells, is the true indicator of the concentration of living microorganisms in the sample. Measuring living biomass through the cATP parameter includes measuring culturable, non-culturable and difficult to culture bacteria.  

Increase of intracellular ATP in samples indicates proliferation of microorganisms and a quality degradation of complex organic samples. If active biomass reaches levels above recommended thresholds, a microbiological drift is taking place. Increase of the active biomass can be seen as an early alarm of microbial disorders in the installation.

Principle of the analysis

Organic compounds are washed from the sample for their elimination. The sample is submitted to filtration through a filter attached to a syringe (QGO Prep kit). Only living micro-organisms are retained by the filter’s porosity. Then, lysis of microorganisms is performed directly on the filter using the UltraLyse™ reagent and intracellular ATP is released. The ATP solution is then diluted and the concentration of ATP is measured using Luminase™ reagent. An external standard calibrated ATP solution, Ultracheck™ 1, allows providing reliable quantitative results.

Total time to result is 3 minutes. Final results are delivered either in pg ATP/ml or Equivalent Microorganisms/ mL (1pg ATP = 1000 Equivalent microorganisms). Equivalent microorganisms include culturable microorganisms and non culturable microorganisms and this level is not directly comparable to CFU results from culture methods.

What are the differences between QGO-M XLPD and QGO-M?

QGO-M XLPD is available for samples that are more difficult to filter and/or have large quantities of inhibitory components (such as latex, polymers, concrete admixtures and personal care products). Therefore, QGO-M XLPD contains same components as QGO-M but QGO-M XLPD includes also 1/10 initial pre-dilution of the sample and standard Luminase™ is replaced by LuminaseXL (10 times stronger than standard Luminase).

QGO-M XLPD kit components

Components for 50 tests	Store at (°C)	Shelf-life
Luminase™XL Dropper Luciferase Enzyme Reagent, 2,5mL	4°C	4 months
UltraCheck 1™  Dropper 1ng ATP/ml Standard, 5mL	20°C	12 months
UltraLyse 7™ Bottle cATP Extraction Reagent, 125mL	20°C	12 months
UltraLute™ Bottle cATP Dilution Reagent, 500mL	20°C	12 months
LumiClean™ Bottle Filter Wash Reagent, 500mL	20°C	12 months
QGO-M Pre-Dilution Tubes cATP Dilution Reagent, 9mL, 50/rack	20°C	12 months

QGO-prep kit content

100 x 20mL Luer-Lock syringes

5 x 60mL Luer-Lock syringes

100 x 25 mm filters for syringes