QGA™ - Quench-Gone Aqueous

Quench-Gone™ Aqueous (QGA™) test kits are designed to measure biological activity in most low-solids water types. Use QGA to quantify total living microorganisms in various water samples, including:

Surface water Potable water Sanitary water

Ultrapure water Cooling tower water Industrial Process Water

Validation of water disinfection Ground water Recycled water

QGA kit measures only Intracellular ATP (cATP) and allows direct quantification of all living microorganisms. Intracellular ATP is the ATP content in living cells. This is the true indicator of total viable flora. Measuring living biomass through the quantification of cATP parameters includes quantification of cultivable, difficult to culture and non cultivable microorganisms.

Increase of Intracellular ATP in water samples indicates proliferation of microorganisms and degradation of water quality. If active biomass reaches levels above recommended thresholds, a microbiological drift is taking place. Consequences are possible proliferation of pathogenic bacteria and increase of sanitary risk. Increase of active biomass levels can be a part of an early alarm for microbial disorders in the installations. 

Principle of the analysis

The water sample (50ml in most cases) is submitted to filtration through a filter attached to a syringe (QG-prep kit). Only living micro-organisms are retained by the filter’s porosity. Then, lysis of microorganisms is performed directly on the filter using UltraLyse 7 reagent and Intracellular ATP is released. The ATP solution is then diluted and the concentration of ATP is measured using Luminase reagent (Luciferine/Luciferase complex). An external standard calibrated ATP solution, Ultracheck1, allows providing reliable quantitative results. Total time to result is 3 minutes. Final results are delivered either in pg ATP/ml of Equivalent Microorganismes/ ml (1pg ATP = 1000 Equivalent microorganisms). Equivalent microorganisms include cultivable microorganisms and non cultivable microorganisms and this level is not directly comparable to CFU results from culture methods.

QGA kit components

Components for 100 tests	Storage	Shelf-life
Luminase™ Dropper Luciferase Enzyme Reagent, 5mL	4°C	4 months
UltraCheck 1™ Dropper 1 ng ATP/mL Standard, 5mL	20°C	12 months
UltraLyse 7™ Bottle cATP Extraction Reagent, 125mL	20°C	12 months
UltraLute™ Bottle cATP Dilution Reagent, 500mL	20°C	12 months

 

QGA-prep kit content

100 x 60mL Luer-Lock syringes

100 x 25 mm filters for syringes