QG21W™ - Quench-Gone™ Wastewater

How and where using this product?

The QG21W kit is designed for detecting biological activity in the field of biological waste water treatment and in samples containing a lot of suspended solids. Use the QG21W kit in order to quantify living microorganisms and assess the active biomass concentration and health in lots of types of water and sludge such as:

Activated sludge Aerobic, anaerobic, anoxic reactors Membrane bioreactors Lagoons Toxicity studies Influents and effluents UASB reactor

Anaerobic Digestion methanogenesis  Bio-fermentation Bio-remediation Composting Individual sewage treatment

The 2^nd generation ATP-metry technology is the only one allowing interference-free quantification of living biomass in samples as complex as those from biological waste water treatment plants. The kit measures all aerobic, anaerobic, anoxic, cultivable or non cultivable microorganisms.

Principle of the analysis

The QG21W kit measures total ATP and extra-cellular ATP in water samples, activated sludge samples or any other kind of samples from a biological waste water treatment plant.

• Total ATP (tATP) is the sum of Intracellular ATP and Extra-cellular ATP.
• Extra-cellular ATP (dATP) is the ATP present in the environment outside living biological cells. dATP is released when microorganisms die.  

Using these two measures, the following biological waste water treatment monitoring parameters are calculated:

Intracellular ATP – cATP

Intracellular ATP is ATP contained within living biological cells and is representative of the concentration of all living microorganisms in a sample.
cATP™ is calculated as the difference of dATP™ and tATP™ : cATP = tATP – dATP.
cATP is directly linked to the concentration of active biomass in bioreactors or in the influents and effluents of biological waste water treatment plants.

Biomass Stress Index - BSI™

The Biomass Stress Index (BSI) is representing the living biomass population’s stress and health.
The BSI is defined as the ratio of dATP to tATP: BSI (%) = dATP/tATP.

Hence, in a sample, the higher the dATP content relative to the tATP, the higher the BSI. High BSI values are related to stressful operating conditions leading to an increase of cells mortality. Stresses such as oxygen depravation, toxicity in the influent, or nutrient deficiencies will produce high BSI values. The BSI parameter is a 'general alarm' of  water or wastewater process, where a change in BSI signals a change in biomass quality, for better or worse. In a biological waste water treatment plant, it is better to monitor the BSI parameter and to minimise it. In particular, the BSI parameter is used during toxicity studies of influents which can deteriorate the active biomass function.

Ratio of Active Biomass - ABR™

The parameter ABR defines the percentage of active biomass among total suspended material in the bioreactor.
The ABR corresponds to the ratio between the concentration of alive biomass and the concentration in Total Suspended Solids (TSS): ABR (%) = (cATP™ (ng/mL) * 0,5) / TSS (mg/L).
The availability of this parameter allows the operator to measure and, consequently, to monitor the activity of bio-solids contained in the bioreactor.

QG21W kit components

Components for 50 tests	Store at (°C)	Shelf-life
LuminaseW Dropper Luciferase Enzyme Reagent, 8mL	4°C	4 months
UltraCheck 1™ Dropper 1 ng ATP /mL Standard, 5mL	20°C	12 months
QG21W Extraction Tubes UltraLyse™ 3021, 2mL, 50/rack	20°C	12 months
QG21W Dilution Tubes UltraLute™/Resin, 8mL, 50/rack	20°C	12 months
QG21W LumiSolve Tubes LumiSolve™, 10mL, 50/rack	20°C	12 months