EFFICIENT MEASUREMENT OF
DETECT ALL LIVING MICRO-ORGANISMS
WITH ATP 2G!
Unlike genetic analyses that rely on DNA specificity, or immunological analyses that rely on antigen specificity, ATP measurement does not specifically identify any microbial species. The ATP molecule is common to all living things.
However, ATP-metry generates an extremely reliable indication of the total number of living micro-organisms, also referred to as total biomass, total biological burden, or total active flora.
The range of micro-organisms detectable by ATP 2G technology is among the largest possible, as it can detect 99.9% of micro-organisms found in liquid environments.
One hundred percent of bacteria and other cells that form aggregates or protective matrices (biofilms, mucilage, filaments, vesicles, etc.) can be detected, as well as a wide range of planktonic micro-organisms, including cyanobacteria (Cyanophyceae or blue algae), single-celled or filamentous yeasts, amebae and other protozoans, Dinophyceae and other single-celled algae, membranous or filamentous algae, diatoms, etc.
Only some rare metabolically inactive biological structures (which do not contain ATP) or those with cell walls that are resistant to enzymatic digestion escape analysis. This is the case for viruses or bacterial spores.
The biological burden of a water sample is traditionally determined in a laboratory by analyzing aerobic micro-organisms that grow at 22°C or 36°C on solid media (NF EN ISO 6222). This technique allows quantification of the many aerobic bacteria that are viable and cultivatable under the given conditions (expressed as the number of CFUs or Colony-forming Units). However, many live micro-organisms cannot be quantified using this methodology.
• strict anaerobic bacteria;
• clumping or aggregate-forming bacteria ou agrégées (which are counted as 1 CFU);
• stressed or dormant bacteria;
• viable bacteria that cannot be cultured (VBNC - Viable but nonculturable) which represent close to 99% of bacterial species known to date!
• most micro-organisms that form biofilms or are protected by some other cellular structure;
• most planktonic micro-organisms, which do not grow well under typical culturing conditions.
Traditional analyses using solid growth media therefore often underestimate the actual level of contamination, leading to inappropriate, often risky, decision-making.