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Cleaning and disinfection in industrial (CIP - Cleaning in Place) and water-based facilitates represent a significant user burden and a not insignificant ecological cost (managing effluents, consumption of energy, water, detergents, biocides, etc.). Optimizing these procedures is necessary for:
• improving economic performance: reducing the consumption of energy, water, detergents, biocidal agents; minimizing cleaning-disinfection time to make equipment available more quickly;
• improving environmental performance: reducing polluting effluents and carbon impact; implementing new ecological treatment strategies.
Rapid microbiological analysis tools like ATP 2G improve the targeting of cleaning-disinfection procedures and help the user minimize the time needed for:
• real-time tracking of water (or fluid) quality within a facility to detect any biological shifts (by monitoring critical control points within the network);
• monitoring biofilm or microbial colony development within a facility;
• optimizing cleaning or disinfection processes to reduce to dose of biocidal agent or detergent needed, as well as reducing energy consumption, duration of treatment or the portion of the installation that needs to be treated;
• following wash, rinse, and purge processes in real time to improve their efficiency and output;
• verifying the effectiveness of biocidal agents, whether chemical or new alternatives for ecological treatment (for example, enzymatic disinfection).
• A biofilm is a microbial population that is protected by an organic matrix and can adhere to surfaces (equipment, pipes, filters, exchangers, reservoirs, etc.). Many bacterial species can form biofilms.
• Biofilms pose problems in industry (notably in closed circuits that must be cleaned in place by CIP procedures) because they are invisible and resistant to disinfection (the matrix makes them particularly resistant to detergents and disinfectants) and promote the establishment of pathogenic organisms within the system.
• There are some effective enzymatic treatments that can break down the matrix and eliminate biofilms during rinsing.
• Analyzing rinse water by ATP-metry can verify the success of the disinfection process.
Cells can lose ATP to the extracellular environment when subjected to environmental stress (as extreme physico-chemical conditions increase the permeability of the cell wall). Cellular stress can be caused by treatment with disinfectants, heat or chemicals. Cell stress differs from cell lysis in that the cell remains alive. Certain kits marketed by aqua-tools Rapid Microbial Solutions (QGA21 2G Refill) allow separate quantification of extracellular ATP (released by dead or stressed cells and present in a dissolved form in water) and total ATP (cellular and extracellular), which can be used to calculate the Biomass Stress Index™ (BSI). This numeric indicator (in %), which corresponds to the proportion of extracellular ATP to total ATP, describes the level of cell death and stress in the biomass present in a facility (or involved in a process). The higher the BSI, the greater the cellular stress and/or death. This index can be used to evaluate the effectiveness of a disinfection treatment or to monitor the health status of a biofilm or contaminant in a closed network.Return to ATP 2G